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Literature summary extracted from

  • Han, Q.; Zhou, C.; Wu, S.; Liu, Y.; Triplett, L.; Miao, J.; Tokuhisa, J.; Deblais, L.; Robinson, H.; Leach, J.E.; Li, J.; Zhao, B.
    Crystal structure of Xanthomonas AvrRxo1-ORF1, a type III effector with a polynucleotide kinase domain, and its interactor AvrRxo1-ORF2 (2015), Structure, 23, 1900-1909 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.236 expressed in Escherichia coli C41 cells and Xanthomonas oryzae strain X11-5A Xanthomonas oryzae pv. oryzicola

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.236 AvrRxo1-ORF1:AvrRxo1-ORF2 tetramer complex, hanging drop vapor diffusion method, using either 0.8 M NaH2PO4, 0.8 M KH2PO4, 15% (v/v) glycerol, 8 mM dithiothreitol, and 0.1 M HEPES pH 7.5 (native or selenomethione-labeled enzyme) or 1.6 M (NH4)2SO4, 18% (v/v) glycerol, 8 mM dithiothreitol, 100 mM Na-citrate pH 5.0 (sulfate complex) Xanthomonas oryzae pv. oryzicola

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.236 T167N ATP-binding site mutant Xanthomonas oryzae pv. oryzicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.236 ATP + NAD+ Xanthomonas oryzae pv. oryzicola
-
 ADP + 3'-NADP+
-
 ?
2.7.1.236 ATP + NAD+ Xanthomonas oryzae pv. oryzicola BLS256
-
 ADP + 3'-NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.236 Xanthomonas oryzae pv. oryzicola Q6TKR8
-
-
2.7.1.236 Xanthomonas oryzae pv. oryzicola BLS256 Q6TKR8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.236 glutathione Sepharose 4B affinity resin Q-Sepharose ion-exchange column chromatography Xanthomonas oryzae pv. oryzicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.236 ATP + NAD+
-
 Xanthomonas oryzae pv. oryzicola ADP + 3'-NADP+
-
 ?
2.7.1.236 ATP + NAD+
-
 Xanthomonas oryzae pv. oryzicola BLS256 ADP + 3'-NADP+
-
 ?
2.7.1.236 additional information the enzyme does not possess uridine diphosphate-N-acetylglucosamine kinase activity Xanthomonas oryzae pv. oryzicola ?
-
-
2.7.1.236 additional information the enzyme does not possess uridine diphosphate-N-acetylglucosamine kinase activity Xanthomonas oryzae pv. oryzicola BLS256 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.236 AvrRxo1
-
 Xanthomonas oryzae pv. oryzicola

General Information

EC Number General Information Comment Organism
2.7.1.236 physiological function the enzyme has a significant virulence function that can increase bacterial growth on the host plant. The enzyme functions to enhance bacterial multiplication in plants, and full virulence function is dependent on its putative kinase activity Xanthomonas oryzae pv. oryzicola